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Efficient molecular sexing in dioecious Silene latifolia and S. dioica and paternity analysis in F 1 hybrids
Author(s) -
HOBZA R,
WIDMER A
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02344.x
Subject(s) - biology , sexing , silene , polymerase chain reaction , interspecific competition , interspecific hybrids , botany , genetics , hybrid , gene
Two polymerase chain reaction‐based assays have been developed that work in combination with an efficient DNA extraction protocol to rapidly and reliably determine sex in the dioecious plant species Silene latifolia and S. dioica . In addition, one of the assays allows assessing paternity in the F 1 generation of intra‐ and interspecific matings involving the two species.