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Isolation and characterization of microsatellite markers in the lowland leopard frog ( Rana yavapaiensis ) and the relict leopard frog ( R. onca ), two declining frogs of the North American desert southwest
Author(s) -
SAVAGE ANNA E,
JAEGER JEF R
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02343.x
Subject(s) - biology , leopard frog , microsatellite , leopard , linkage disequilibrium , locus (genetics) , zoology , population , allele , evolutionary biology , ecology , genetics , haplotype , amphibian , gene , demography , sociology
We characterized 15 microsatellite loci for the lowland leopard frog ( Rana yavapaiensis ) and the relict leopard frog ( R. onca ) for future studies of population genetic structure and relatedness. Analysis of 20 individuals from single populations of each species showed that all markers were polymorphic in at least one species. Observed and expected heterozygosities ranged from 0 to 0.94 and from 0.11 to 0.85, respectively, and there were three to 11 alleles per locus. No loci were in linkage disequilibrium, but six loci deviated significantly from Hardy–Weinberg equilibrium, and the presence of a null allele was detected in two of these loci.