Premium
Development and characterization of novel, polymorphic microsatellite markers for oat crown rust, Puccinia coronata
Author(s) -
DAMBROSKI HATTIE R,
CARSON MARTIN L
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02336.x
Subject(s) - biology , loss of heterozygosity , microsatellite , locus (genetics) , genetics , allele , null allele , population , genetic diversity , gene , demography , sociology
We report the development of 37 novel and polymorphic microsatellite markers for oat crown rust, Puccinia coronata f.sp. avenae . The allelic diversity ranged from two to 16 alleles per locus. Observed heterozygosity ranged from 0.000 to 0.971, and expected heterozygosity from 0.057 to 0.848. Thirteen of the loci were not in Hardy‐Weinberg equilibrium, due to either the presence of null alleles, small sample size, or the effects of population subdivision (Wahlund's effect). All 37 primer pairs were tested with P. graminis and P. triticina showing that they are specific to P. coronata .