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Isolation and characteristics of 10 microsatellite markers from the endangered coconut crab ( Birgus latro )
Author(s) -
GAN CHAIHSIA,
TEE SEEMIN,
TANG PEICIAO,
YANG JAY MINGCHE,
FREIRE FRANCIS,
MCGOWAN ANDREW,
NARRIMAN JIDDAWI,
MOHAMMED MOHAMMED SULEIMAN,
HSIEH HWEYLIAN,
CHEN CHANGPO,
SHEPPARD CHARLES,
CHEN CHAOLUN ALLEN
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02330.x
Subject(s) - biology , microsatellite , endangered species , evolutionary biology , genetic diversity , locus (genetics) , allele , zoology , population , genetics , ecology , gene , demography , sociology , habitat
The coconut crab ( Birgus latro ), an endangered marine‐dispersed crustacean, is facing severe and probably accelerating population extinction worldwide, but biological information on its conservation remains deficient. In order to reveal the genetic structure of B. latro , 10 microsatellite loci were developed. A high degree of polymorphism was observed with a mean number of alleles per locus of 16.9. The mean expected heterozygosities were also high, ranging from 0.742 to 0.965. The observed heterozygosities ranged from 0.210 to 0.925. Departures from Hardy–Weinberg equilibrium were observed at five loci after the Bonferroni correction. These hypervariable markers will be utilized to study the genetic diversity and conservation of B. latro throughout its distribution range in the Pacific and Indian Oceans.