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Isolation and characterization of microsatellite loci in Ainsliaea faurieana (Asteraceae), an endemic plant species on Yakushima Island, Japan, and cross‐species amplification in closely related taxa
Author(s) -
MITSUI Y.,
ISAGI Y.,
SETOGUCHI H.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02325.x
Subject(s) - biology , asteraceae , taxon , microsatellite , isolation (microbiology) , botany , evolutionary biology , ecology , genetics , gene , bioinformatics , allele
Fourteen microsatellite loci were isolated and characterized for Ainsliaea faurieana , an endemic perennial plant species on Yakushima Island. In an analysis of 37 individuals from Yakushima Island, the observed number of alleles ranged from two to six. The expected and observed heterozygosities were 0.109–0.811 and 0.000–0.27, respectively. The inbreeding coefficient was 0.489–1.000. The interspecific applicability of these microsatellites was evaluated by analysing three closely related taxa from the Ryukyu Islands. All primer pairs for the 14 loci tested successfully amplified in all taxa.