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Development and characterization of 12 polymorphic microsatellite loci in the American dog tick ( Dermacentor variabilis )
Author(s) -
DHARMARAJAN GUHA,
FIKE JENNIFER A.,
BEASLEY JAMES C.,
RHODES JR OLIN E.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02313.x
Subject(s) - dermacentor variabilis , biology , microsatellite , locus (genetics) , tick , allele , gene flow , genetics , zoology , gene , ixodidae , genetic variation , virology
Twelve polymorphic microsatellite loci were developed for the American dog tick ( Dermacentor variabilis ), an important vector of infectious diseases in humans and animals. Four multiplexed panels comprising the loci were developed and 45 ticks collected from two raccoons ( Procyon lotor ) were genotyped. The number of alleles per locus ranged from nine to 30, and single locus heterozygosities ranged from 0.18 to 0.93. Data generated using these markers will further our understanding of factors affecting gene flow in D. variabilis , thus helping to elucidate the transmission dynamics of diseases associated with this vector.