Premium
Isolation and characterization of polymorphic microsatellite loci in the green leafhopper Empoasca vitis Goethe (Homoptera)
Author(s) -
PAPURA D.,
GIRESSE X.,
CHAUVIN B.,
CARON H.,
DELMOTTE F.,
VAN HELDEN M.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02298.x
Subject(s) - biology , leafhopper , microsatellite , locus (genetics) , homoptera , allele , genetics , genetic diversity , population , botany , hemiptera , gene , pest analysis , demography , sociology
Eight dinucleotide microsatellite loci were isolated and characterized within the green leafhopper Empoasca vitis (Goethe) using an enrichment cloning procedure. Primers were tested on 171 individuals collected in the southwest of France from the vine plants. The identified loci were polymorphic, with allelic diversity ranging from two to 18 alleles per locus. Observed heterozygosities were from 0.021 to 0.760. These microsatellite markers should prove to be a useful tool for estimating the population genetic structure, host‐plant specialization and migration capacity of this insect.