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Species identification of aphids (Insecta: Hemiptera: Aphididae) through DNA barcodes
Author(s) -
FOOTTIT R. G.,
MAW H. E. L.,
VON DOHLEN C. D.,
HEBERT P. D. N.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02297.x
Subject(s) - biology , aphididae , dna barcoding , hemiptera , mitochondrial dna , zoology , nadh dehydrogenase , botany , genetics , barcode , homoptera , pest analysis , gene , computer science , operating system
A 658‐bp fragment of mitochondrial DNA from the 5′ region of the mitochondrial cytochrome c oxidase 1 (COI) gene has been adopted as the standard DNA barcode region for animal life. In this study, we test its effectiveness in the discrimination of over 300 species of aphids from more than 130 genera. Most (96%) species were well differentiated, and sequence variation within species was low, averaging just 0.2%. Despite the complex life cycles and parthenogenetic reproduction of aphids, DNA barcodes are an effective tool for identification.