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Isolation and characterization of microsatellite markers for Carolina hemlock ( Tsuga caroliniana )
Author(s) -
JOSSERAND S. A.,
POTTER K. M.,
ECHT C. S.,
NELSON C. D.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02294.x
Subject(s) - tsuga , biology , microsatellite , ecology , allele , genetics , gene
We describe the isolation and characterization of 31 polymorphic di‐ and trinucleotide microsatellite marker loci for Carolina hemlock ( Tsuga caroliniana Englem.). In addition, primer pairs for 16 loci amplified scoreable alleles in six other Tsuga species. In eastern North America, both Carolina hemlock and eastern hemlock ( Tsuga canadensis [L.] Carr.) populations are declining due to infestation by hemlock woolly adelgid, Adelges tsugae . The markers described here should enhance population genetic studies of hemlocks, providing valuable information for conserving and restoring these important forest tree species.