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Development of simple sequence repeat markers from expressed sequence tags of the black tiger shrimp ( Penaeus monodon )
Author(s) -
WHANKAEW SUKHUMAN,
TANGPHATSORNRUANG SITHICHOKE,
TRIWITAYAKORN KANOKPORN
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02290.x
Subject(s) - penaeus monodon , biology , shrimp , primer (cosmetics) , genetics , tiger , locus (genetics) , expressed sequence tag , allele , population , fishery , gene , complementary dna , chemistry , demography , computer security , organic chemistry , sociology , computer science
In this study, we describe the development of expressed sequence tag–simple sequence repeat markers from expressed sequence tags of the black tiger shrimp ( Penaeus monodon ) deposited in public sequence databases. A total of 46 primer pairs were designed and screened on 26 individuals of P. monodon from a natural population. Of these, 16 primer pairs showed polymorphic profiles with between two and five alleles per locus. The average unbiased and direct count heterozygosities were 0.4662 and 0.3516, respectively. Cross‐amplification was tested with five individuals of Penaeus vannamei and polymorphic products were detected at five loci.