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Isolation and characterization of microsatellite markers for the tree‐root endophytes Phialocephala subalpina and Phialocephala fortinii s.s.
Author(s) -
QUELOZ VALENTIN,
DUÒ ANGELO,
GRÜNIG CHRISTOPH R.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02279.x
Subject(s) - biology , microsatellite , species complex , pinaceae , plant use of endophytic fungi in defense , botany , genetics , phylogenetic tree , gene , pinus <genus> , allele
Species of the Phialocephala fortinii s.l.– Acephala applanata complex are the dominant dark septate endophytes (DSE) in roots of species belonging to the Pinaceae . The two species Phialocephala subalpina and P. fortinii s.s. belong to the most widely distributed species within this complex. In the present study, 15 polymorphic microsatellite loci were developed for these two closely related species. Strains of a community which were analysed previously using single‐copy restriction fragment length polymorphism were screened with the new markers. Microsatellites were suitable to classify the two species and to recognize individuals within species.