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Isolation and characterization of 12 polymorphic microsatellite markers in the tropical house gecko ( Hemidactylus mabouia )
Author(s) -
SHORT KRISTEN HARFMANN,
PETREN KENNETH
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02270.x
Subject(s) - biology , gecko , microsatellite , null allele , population , genetics , allele , polymerase chain reaction , zoology , evolutionary biology , gene , demography , sociology
We present 12 variable microsatellite loci isolated from the invasive tropical house gecko, Hemidactylus mabouia . Polymerase chain reaction (PCR) primers were tested on 39 individuals from two locations in Miami, Florida. Heterozygote deficiency was detected for four loci, and we attribute this to possible null alleles or population substructure. Some loci successfully amplified PCR products in several congeners, indicating their potential for use in other geckos.