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Development of microsatellite markers for the wood cricket, Nemobius sylvestris (Orthoptera: Gryllidae)
Author(s) -
VANHALA TYTTI,
COTTRELL JOAN
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02269.x
Subject(s) - cricket , biology , microsatellite , orthoptera , gryllus bimaculatus , field cricket , genus , evolutionary biology , zoology , allele , genetics , gene
Thirty‐four novel microsatellite markers developed for wood cricket ( Nemobius sylvestris ) were tested and optimized. Twenty‐five microsatellite loci were polymorphic, exhibiting between two and nine alleles. Observed heterozygosities ranged from 0.038 to 0.925. The microsatellites were also tested in a species belonging to another genus of the Gryllidae family ( Gryllus bimaculatus ). Two markers produced clear banding patterns with the expected product size. These markers will be used to study the effects of forest fragmentation on genetic connectivity using wood cricket as a model species.