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Isolation and characterization of microsatellite markers from the phytopathogenic fungus Alternaria dauci
Author(s) -
BENICHOU SOUMAYA,
DONGO ANITA,
HENNI DJAMEL EDDINE,
PELTIER DIDIER,
SIMONEAU PHILIPPE
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02237.x
Subject(s) - biology , microsatellite , alternaria , alternaria solani , allele , fungus , fungi imperfecti , amplicon , botany , genetics , polymerase chain reaction , gene , blight
Eleven polymorphic microsatellite markers were isolated from the necrotrophic phytopathogenic fungus Alternaria dauci based on enriched genomic libraries. In order to assess allelic variability, the microsatellite loci were analysed in a collection of 43 isolates. The number of detected alleles in 11 loci ranged from two to 24 (mean 10.4). Test of cross‐species amplification and sequencing of the resulting amplicons showed that some of these microsatellites could be used in different species such as Alternaria solani , Alternaria bataticola and Alternaria zinniae .