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Tetranucleotide microsatellite loci from the black bear ( Ursus americanus )
Author(s) -
SANDERLIN JAMIE SKVARLA,
FAIRCLOTH BRANT C.,
SHAMBLIN BRIAN,
CONROY MICHAEL J.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02234.x
Subject(s) - ursus , biology , microsatellite , polymerase chain reaction , primer (cosmetics) , range (aeronautics) , loss of heterozygosity , genetics , allele , ursus maritimus , zoology , ecology , population , demography , gene , chemistry , materials science , organic chemistry , sociology , composite material , arctic
We describe primers and polymerase chain reaction conditions to amplify 21 tetranucleotide microsatellite DNA loci in black bears ( Ursus americanus ). We tested primers using individuals from two populations, one each in Georgia and Florida. Among individuals from Georgia ( n = 29), primer pairs yielded an average of 2.9 alleles (range, one to four) and an average observed heterozygosity ( H O ) of 0.50 (range, 0.00 to 0.79). Among individuals from Florida ( n = 19), primer pairs yielded an average of 5.7 alleles (range, one to 14) and an H O of 0.55 (range, 0.00 to 1.00). A comparison of previously developed markers with individuals from Georgia suggests that bear populations in Georgia and Florida have reduced allelic diversity relative to other populations.