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Development of microsatellite markers for the bracken fern, Pteridium aquilinum
Author(s) -
CHEN XIAOQING,
WANG JIANXIU,
TIAN HONGLI,
ZHANG XIANCHUN,
WEN JUN,
ZHOU SHILIANG
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02233.x
Subject(s) - pteridium aquilinum , biology , microsatellite , linkage disequilibrium , locus (genetics) , bracken , loss of heterozygosity , genetics , fern , allele , population , haplotype , botany , gene , demography , sociology
We isolated eight novel polymorphic microsatellite loci from Pteridium aquilinum . These loci were characterized in 30 individuals, one from Bolivia, two from Peru, one from the USA, one from Japan, and 25 from Northeast China to Southwest China. The number of alleles per locus ranged from two to seven. The observed heterozygosity ( H O ) ranged from 0.000 to 0.600 with an average of 0.3051, and the expected heterozygosity ( H E ) ranged from 0.0966 to 0.7780 with an average of 0.4267. One locus deviated from Hardy–Weinberg equilibrium and four pairs of loci were found to be in linkage disequilibrium. These polymorphic loci will be useful in the study of the population genetic structure of Pteridium .