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Cross‐species characterisation of polymorphic microsatellite loci in the giant otter ( Pteronura brasiliensis )
Author(s) -
PICKLES ROBERT S. A.,
GROOMBRIDGE JAMES J.,
ROJAS VERONICA D. ZAMBRANA,
JORDAN WILLIAM C.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02232.x
Subject(s) - lutra , otter , biology , microsatellite , loss of heterozygosity , zoology , allele , ecology , genetics , gene
Nineteen microsatellite loci developed for the Eurasian otter ( Lutra lutra ) and 15 loci developed for the North American river otter ( Lontra canadensis ) were tested for ease of amplification and degree of polymorphism on a set of 20 giant otter ( Pteronura brasiliensis ) faecal samples from the Bolivian Amazon basin. Nineteen loci amplified consistently well, with polymorphisms ranging from two to nine alleles and observed heterozygosity ranging from 0.15 to 0.85.