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Isolation and characterization of 22 polymorphic microsatellite loci for the Barrow's goldeneye ( Bucephala islandica )
Author(s) -
JAARI SONJA,
JAATINEN KIM,
MERILÄ JUHA
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02225.x
Subject(s) - biology , microsatellite , locus (genetics) , loss of heterozygosity , genetics , allele , polymerase chain reaction , population , zoology , gene , demography , sociology
We describe primers and polymerase chain reaction conditions to amplify 22 microsatellite loci from the Barrow's goldeneye ( Bucephala islandica ). The primers were tested on 27 individuals from a population breeding in British Columbia, Canada. The developed primer pairs yielded an average of 6.11 alleles per locus (range 2–12), an average observed heterozygosity of 0.70 (range 0.07–0.96) and a polymorphic information content of 0.07–0.88.