Premium
Improved COI barcoding primers for Southeast Asian perching birds (Aves: Passeriformes)
Author(s) -
LOHMAN DAVID J.,
PRAWIRADILAGA DEWI M.,
MEIER RUDOLF
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02221.x
Subject(s) - dna barcoding , biology , passerine , mitochondrial dna , primer (cosmetics) , evolutionary biology , zoology , cytochrome c oxidase subunit i , genetics , gene , chemistry , organic chemistry
The All Birds Barcoding Initiative aims to assemble a DNA barcode database for all bird species, but the 648‐bp ‘barcoding’ region of cytochrome c oxidase subunit I (COI) can be difficult to amplify in Southeast Asian perching birds (Aves: Passeriformes). Using COI sequences from complete mitochondrial genomes, we designed a primer pair that more reliably amplifies and sequences the COI barcoding region of Southeast Asian passerine birds. The 655‐bp region amplified with these primers overlaps the COI region amplified with other barcoding primer pairs, enabling direct comparison of sequences with previously published DNA barcodes.