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Eight polymorphic microsatellite markers developed in the Chinese scorpion, Mesobuthus martensii (Scorpiones: Buthidae)
Author(s) -
JI YAJIE,
LENG LIANG,
SHI CHENGMIN,
HUA YUEPING,
ZHANG DEXING
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02215.x
Subject(s) - biology , microsatellite , linkage disequilibrium , genetics , buthidae , scorpion , loss of heterozygosity , allele , evolutionary biology , haplotype , gene , ecology , venom
Eight polymorphic di‐ and trinucleotide microsatellite loci were developed in the Chinese scorpion, Mesobuthus martensii. The expected heterozygosity at these loci ranges from 0.019 to 0.860, with the observed allele numbers varying from two to 25. Overall, there were no deviations from Hardy–Weinberg equilibrium and no observed linkage disequilibrium after Bonferroni correction. Cross‐species amplification of these loci in Mesobuthus eupeus revealed that five loci can amplify successfully in this species. The polymorphic microsatellite DNA markers reported here should provide helpful means to address questions concerning phylogeographical patterns and evolutionary history of M. martensii and closely related species.