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Development of new microsatellite markers from a salt‐marsh sedge Carex rugulosa by compound simple sequence repeat–polymerase chain reaction
Author(s) -
OHBAYASHI K.,
HODOKI Y.,
NAKAYAMA S.,
SHIMADA M.,
KUNII H.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02212.x
Subject(s) - biology , microsatellite , genetics , carex , locus (genetics) , genetic diversity , polymerase chain reaction , genetic marker , allele , botany , gene , population , demography , sociology
We developed 12 polymorphic microsatellite markers from a salt‐marsh sedge Carex rugulosa. The number of alleles per locus ranged from two to four, with an average of 2.75. The observed and expected heterozygosities ranged from 0.067 to 0.600 and from 0.128 to 0.620, respectively. These simple sequence repeat markers will allow the identification of genets and evaluation of the genetic diversity of C. rugulosa .