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Molecular markers to determine the ecological fate of Bacillus thuringiensis ssp. kurstaki
Author(s) -
SHRINIVAS K.,
KESTER K.,
MARTIN P. A. W.,
BROWN B.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02207.x
Subject(s) - bacillus thuringiensis , subspecies , biology , bruton's tyrosine kinase , primer (cosmetics) , bacillaceae , ecology , bacteria , genetics , bacillus subtilis , signal transduction , chemistry , organic chemistry , tyrosine kinase
A set of DNA markers was developed that successfully identifies Bacillus thuringiensis ssp. kurstaki ( Btk ) when screened against other Bacillus species and subspecies. These subspecies‐specific primer sets allowed detection and characterization of Btk within an environmental background that contained many Bacillus species. Because Btk is used as an active ingredient in many commercial formulations, yet is not naturally widely distributed in North America or Europe, these markers will prove useful in investigations on the environmental persistence and ecological fate of Btk .