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Isolation and characterization of microsatellite markers for the ornamental discus fish Symphysodon discus and cross‐species amplification in other Heroini cichlid species
Author(s) -
AMADO MANUELLA VILLAR,
HRBEK TOMAS,
GRAVENA WALESKA,
FANTIN CLEITON,
DE ASSUNÇÃO ENEDIOGUEIRA,
ASTOLFIFILHO SPARTACO,
FARIAS IZENI P.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02200.x
Subject(s) - biology , microsatellite , cichlid , linkage disequilibrium , locus (genetics) , allele , ornamental plant , genetics , zoology , ecology , fish <actinopterygii> , fishery , haplotype , gene
The discus fishes ( Symphysodon spp.) are economically important ornamental species. Thirteen microsatellite markers were developed from a CT 12 ‐ and CA 12 ‐enriched whole genomic DNA library of Symphysodon discus . Allelic variability was tested on 44 individuals of two species ( S. discus and S. aequifasciatus ). Allelic richness ranged from two to 11 alleles per locus and observed heterozygosities from 0.083 to 0.998. All loci were at Hardy–Weinberg equilibrium, and no pair of loci showed linkage disequilibrium within a species. Cross‐species amplification was also successfully performed in the Neotropical cichlids Uaru amphiacanthoides , Hoplarchus psittacus , Hypselecara coryphaenoides , Pterophyllum sp., Mesonauta sp. and Heros sp.