Premium
Isolation and characterization of nine microsatellite loci from the pike‐perch, Sander lucioperca (Linnaeus, 1758)
Author(s) -
KOHLMANN KLAUS,
KERSTEN PETRA
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02166.x
Subject(s) - biology , microsatellite , locus (genetics) , linkage disequilibrium , genetics , pike , allele , allele frequency , evolutionary biology , haplotype , gene , fishery , fish <actinopterygii>
In order to facilitate studies on the genetic structure of wild populations as well as to monitor genetic changes in cultured stocks, nine polymorphic microsatellite loci were isolated from pike‐perch ( Sander lucioperca ). Single loci allele numbers varied between two (loci MSL‐3 and MSL‐7 ) and six (loci MSL‐1 and MSL‐2 ), and observed heterozygosities ranged from 0.36 (locus MSL‐2 ) to 1.00 (locus MSL‐9 ) in a test panel of 25 individuals. Only one pair of loci ( MSL‐5 and MSL‐8 ) displayed significant linkage disequilibrium after sequential Bonferroni corrections. Hardy–Weinberg tests revealed significant excesses of heterozygotes at three loci ( MSL‐1 , MSL‐7 , and MSL‐9 ).