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Characterization of microsatellite loci in the Hawaiian endemic shrub Schiedea adamantis (Caryophyllaceae) and amplification in related species and genera
Author(s) -
CULLEY T. M.,
WELLER S. G.,
SAKAI A. K.,
PUTNAM K. A.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02161.x
Subject(s) - biology , microsatellite , caryophyllaceae , silene , shrub , genus , primer (cosmetics) , botany , population , endemism , genetics , allele , ecology , gene , chemistry , demography , organic chemistry , sociology
Schiedea adamantis is a rare, perennial shrub endemic to the Hawaiian island of Oahu where it consists of a single population. Using a nonradioactive protocol, 12 microsatellite primers were developed that consisted of di‐, tri‐, penta‐ and hexanucleotide repeats. Using multiplexed reactions, all but two primers exhibited polymorphism with an average of 3.67 alleles per primer. Most primers also amplified in 28 additional Schiedea species, revealing wide applicability across the genus; eight and nine primers also amplified in Honckenya peploides and Silene lanceolata , respectively, related genera in the Caryophyllaceae. This is the first known report of microsatellite primers developed in Schiedea .