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Development of microsatellite markers for the grapevine fungal pathogen Phaeomoniella chlamydospora
Author(s) -
SMETHAM GABRIELLE,
ADES PETER,
FORD REBECCA
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02152.x
Subject(s) - biology , microsatellite , genotype , locus (genetics) , allele , genetic diversity , genetics , genetic marker , population , genomic dna , botany , gene , sociology , demography
Twenty polymorphic microsatellite markers from microsatellite‐enriched genomic DNA of the grapevine fungal pathogen, Phaeomoniella chlamydospora , were developed and characterized. The markers were used to genotype isolates from Australia and from Europe/Eurasia. The number of alleles per locus ranged from two to 11. Gene diversity per locus ranged from 0.08 to 0.63 among Australian isolates, and from 0.2 to 0.77 among isolates from Europe/Eurasia, demonstrating the suitability of these markers for population genetic studies of P. chlamydospora . Eighteen of the 20 markers also amplified a product in the closely related Phaeoacremonium aleophilum .