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Development of eight polymorphic microsatellites for a Zn/Cd hyperaccumulator Sedum alfredii Hance (Crassulaceae)
Author(s) -
HUANG HUIRUN,
SHU WENSHENG,
MAO ZHIBIN,
GE XUEJUN
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02151.x
Subject(s) - biology , hyperaccumulator , microsatellite , crassulaceae , locus (genetics) , linkage disequilibrium , population , allele , genetics , botany , haplotype , gene , ecology , phytoremediation , soil water , demography , sociology
Sedum alfredii is a Zn/Cd hyperaccumulator distributed in East Asia. A total of eight polymorphic microsatellite markers were developed. These loci were screened in 25 individuals from one heavy metal‐tolerant population and one nontolerant population, respectively. The average allele number of these markers was 5.25 per locus, ranging from two to nine. Population‐specific alleles were found at each locus. The observed and expected heterozygosities ranged from 0.000 to 0.640 and from 0.451 to 0.819. Significant deviation from Hardy–Weinberg equilibrium was detected at both the species and the population level. No significant linkage disequilibrium was detected at population level.