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Microsatellite DNA markers in the giant freshwater prawn, Macrobrachium rosenbergii : a tool for genetic analysis
Author(s) -
DIVU DAMODARAN,
KARUNASAGAR IDDYA,
KARUNASAGAR INDRANI
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02148.x
Subject(s) - macrobrachium rosenbergii , biology , microsatellite , prawn , locus (genetics) , population , genetics , allele , zoology , fishery , gene , demography , sociology
Eight microsatellite loci were identified and characterized in the commercially important giant freshwater prawn, Macrobrachium rosenbergii . The microsatellite loci were detected by the random screening for dinucleotide repeat units within a partial genomic library developed for the species with biotinylated probes (CA) 15 , (AT) 15 and (GA) 15 . All the eight loci were found to be polymorphic. The number of alleles and observed heterozygosities per locus ranged between three to 16 and 0.22 to 0.71, respectively. These microsatellite markers will be useful for the conservation and management of wild and cultured stocks and population genetic studies of freshwater prawn.