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Isolation and characterization of 13 microsatellite markers for the viviparous surfperch Ditrema temmincki (Embiotocidae) and cross‐species amplification
Author(s) -
TAKAGI MOTOHIRO,
SAKAI KUMIKO,
TANIGUCHI NOBUHIKO
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02145.x
Subject(s) - biology , loss of heterozygosity , microsatellite , locus (genetics) , genetics , allele , evolutionary biology , gene
Thirteen microsatellite loci were isolated from a size‐selected genomic library of the surfperch ( Ditrema temmincki Bleeker). All loci displayed a high degree of length polymorphism, as observed in the total number of alleles per locus (two to 23) and a high degree of estimated heterozygosity, ranging from 0.080 to 0.893. The primers developed for D. temmincki were also tested for their ability to amplify homologous sequences in D. viride and Neoditrema ransonetii . Distinct differences were observed among three species of surfperches, in both genetic variability and the frequency distribution of the alleles.