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New microsatellite loci isolated from the field cricket Gryllus bimaculatus characterized in two cricket species, Gryllus bimaculatus and Gryllus campestris
Author(s) -
BRETMAN AMANDA,
DAWSON DEBORAH A.,
HORSBURGH GAVIN J.,
TREGENZA TOM
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02139.x
Subject(s) - gryllus bimaculatus , biology , field cricket , cricket , orthoptera , microsatellite , botany , zoology , genetics , allele , gene
We have developed a new set of 27 polymorphic markers for each of two cricket species, Gryllus bimaculatus and Gryllus campestris . Initially, 14 published G. bimaculatus loci were tested in G. campestris ; however, only five loci were polymorphic. Therefore, we isolated an additional 50 new microsatellite loci from G. bimaculatus and tested these in both species. In a minimum of 20 individuals, 27 of the new loci were polymorphic in G. bimaculatus and 25 in G. campestris .