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Development of nine polymorphic microsatellite loci in the spined loach, Cobitis taenia, and cross‐species amplification in the related species C. elongatoides , C. taurica and C. tanaitica
Author(s) -
DE GELAS K.,
JANKO K.,
VOLCKAERT F. A. M.,
DE CHARLEROY D.,
VAN HOUDT J. K. J.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02135.x
Subject(s) - cobitidae , biology , taenia , microsatellite , zoology , population , allele , genetics , evolutionary biology , helminths , gene , fish <actinopterygii> , fishery , demography , sociology
Abstract Nine polymorphic microsatellite loci were developed for the spined loach, Cobitis taenia (Teleostei: Cobitidae). The loci were validated using 50 individuals from a population in Belgium. Moderate to high levels of polymorphism were detected (two to 11 alleles). In addition, most markers amplified successfully in three closely related taxa that are known to hybridize with C. taenia : C. elongatoides , C. taurica and C. tanaitica. Some of the loci are most likely diagnostic among species. These markers will be valuable for the study of the historical and contemporary interactions within C. taenia and the Cobitis species complex.