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Development of microsatellite markers in a large perennial herb, Veratrum album ssp. oxysepalum
Author(s) -
KATO Y.,
ARAKI K.,
KUBOTA S.,
OHARA M.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02133.x
Subject(s) - biology , microsatellite , locus (genetics) , genetic diversity , allele , genotype , genomic dna , genetics , polymerase chain reaction , population , botany , dna , gene , demography , sociology
We developed 11 polymorphic microsatellite [simple sequence repeat (SSR)] loci from genomic DNA of Veratrum album ssp. oxysepalum using a dual‐suppression polymerase chain reaction technique and an improved method. These markers, with four to 17 alleles per locus, identified 47 genotypes in 48 samples collected from a population in Hokkaido, Japan. The observed and expected heterozygosities ranged from 0.042 to 0.938 and from 0.448 to 0.909, respectively. These SSR markers will be available to identify genets and evaluate genetic diversity of V. album ssp. oxysepalum .