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Isolation and characterization of microsatellite loci in the deep‐sea marine fish, the roundnose grenadier ( Coryphaenoides rupestris )
Author(s) -
KNUTSEN HALVOR,
LE GOFFVITRY MARIE,
FIANI DAVID,
HOELZEL A. RUS
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02132.x
Subject(s) - biology , microsatellite , gadus , loss of heterozygosity , zoology , locus (genetics) , population , polymerase chain reaction , atlantic cod , allele , fishery , genetics , fish <actinopterygii> , gene , demography , sociology
We developed polymerase chain reaction primers for eight dinucleotide microsatellite loci in the marine deep sea fish, roundnose grenadier ( Coryphaenoides rupestris ). All markers were obtained from a partial genomic DNA library, and characterized in 90 unrelated individuals from one putative population sampled on the Mid‐Atlantic Ridge. The number of alleles ranged from two to 61 with an average of 21 per locus. The observed heterozygosity levels ranged from 0.301 to 0.987 with an average of 0.672. Several of the markers amplified multiple alleles from either the Atlantic cod ( Gadus morhua ) or the deep‐sea fish roughhead grenadier ( Macrourus berglax ).