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Newly developed polymorphic microsatellite markers for frogs of the genus Ascaphus
Author(s) -
SPEAR STEPHEN F.,
BAUMSTEIGER JASON,
STORFER ANDREW
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02123.x
Subject(s) - biology , microsatellite , loss of heterozygosity , locus (genetics) , allele , genetics , evolutionary biology , genetic marker , genus , zoology , gene
Thirteen polymorphic microsatellite loci were identified and developed for the coastal tailed frog, Ascaphus truei , from sites within the Olympic Peninsula of Washington, USA. These tetranucleotide repeat loci were highly variable, averaging 19 alleles per locus and expected heterozygosity of 0.91. In addition, these loci cross‐amplify in the sister species, Ascaphus montanus . These markers will prove useful in identifying fine‐scale genetic structure, as well as provide insight into the evolution and conservation of this group across fragmented landscapes.