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New polymorphic microsatellite markers for the limpet Patella rustica and cross‐priming testing in four Patella species
Author(s) -
PÉREZ M.,
LLAVONA A.,
PRESA P.,
RIBEIRO P.,
HAWKINS S. J.,
SANTOS A. M.,
ALEXANDRINO P.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02119.x
Subject(s) - biology , microsatellite , loss of heterozygosity , limpet , locus (genetics) , intraspecific competition , population , patella , zoology , allele , genetics , anatomy , demography , gastropoda , sociology , gene
Eighteen microsatellite markers have been characterized from the Patella rustica genome. An average of 10 alleles per locus and an expected heterozygosity ranging from 0.054 to 0.937 were observed in a sample of 32 wild individuals from Viana do Castelo, Portugal. Distinct cross‐priming amplification rates were recovered on four additional Patella species. Three monomorphic P. rustica loci were polymorphic in other Patella species. The microsatellites developed herein could be a useful intraspecific genetic tool to undertake fine population studies in the genus Patella .