Premium
Development of highly polymorphic tetranucleotide microsatellite markers in Austrocedrus chilensis
Author(s) -
ARANA MARÍA VERÓNICA,
BUONAMICI ANNA,
SEBASTIANI FEDERICO,
ALIA RICARDO,
GALLO LEONARDO A.,
MARCHELLI PAULA,
MORENO CAROLINA,
VENDRAMIN GIOVANNI G.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02099.x
Subject(s) - biology , microsatellite , linkage disequilibrium , loss of heterozygosity , locus (genetics) , genetics , allele , gene flow , genetic marker , population , evolutionary biology , gene , genetic variation , haplotype , demography , sociology
An enriched genomic library was constructed and 9 novel polymorphic tetranucleotide microsatellite markers developed for Austrocedrus chilensis , the most economically important native conifer in the Andean Patagonian region. Polymorphism was investigated for these markers in 48 individuals from two populations. Numbers of alleles ranged from 3 to 19 and levels of observed heterozygosity among the 9 loci ranged from 0.32 to 0.95. No locus combinations exhibited linkage disequilibrium. These polymorphic markers will be useful tools for the study of demography and gene flow and more in general for population and conservation genetics of this species.