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Isolation and characterization of polymorphic microsatellite and COI loci from the whelk Kelletia kelletii
Author(s) -
WHITE CROW,
TOONEN ROBERT J.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02097.x
Subject(s) - biology , microsatellite , multiplex polymerase chain reaction , mitochondrial dna , genetics , multiplex , polymerase chain reaction , hypervariable region , evolutionary biology , cytochrome c oxidase subunit i , gene , allele
There is considerable interest in the genetic structure of Kelletia kelletii because of its economic, ecological and scientific importance. To that end, we developed species‐specific primers which amplify mitochondrial cytochrome c oxidase subunit I as well as 13 hypervariable nuclear microsatellite loci. Using dye‐labelled primers, the microsatellite loci can be co‐amplified in two multiplex polymerase chain reactions and scored simultaneously on an automated sequencer.