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Fifteen new microsatellite markers for the reef coral Favia fragum and a new Symbiodinium microsatellite
Author(s) -
CARLON DAVID B.,
LIPPÉ CATHERINE
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02095.x
Subject(s) - biology , microsatellite , locus (genetics) , symbiodinium , linkage disequilibrium , scleractinia , genetics , zooxanthellae , allele , evolutionary biology , coral , ecology , haplotype , cnidaria , gene , symbiosis , bacteria
Sixteen new microsatellite loci were isolated from the Tropical Atlantic coral Favia fragum . One locus amplified with pure zooxanthellae DNA template, revealing a symbiont ( Symbiodinium ) origin. We genotyped 48 short and 45 tall ecomorphs of F. fragum from the Bocas del Toro region of Panama. For 15 host loci, allelic diversity ranged from three to 23 with an average of 5.75 alleles per locus. Analysis of genotypic data revealed significant heterozygote deficits at all loci and linkage disequilibrium between loci, as did a previous study of the two ecomorphs with allozymes. We found evidence for null alleles at four of the host loci in the form of locus‐specific polymerase chain reaction failure; however, extreme inbreeding via self‐fertilization is likely to explain the large departures from Hardy–Weinberg equilibrium.