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Isolation and characterization of microsatellite markers from the marine isopods Serolis paradoxa and Septemserolis septemcarinata (Crustacea: Peracarida)
Author(s) -
LEESE FLORIAN,
KOP ANNA,
AGRAWAL SHOBHIT,
HELD CHRISTOPH
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2007.02078.x
Subject(s) - biology , microsatellite , loss of heterozygosity , locus (genetics) , genetics , allele , population , zoology , gene , demography , sociology
This study reports the successful isolation of highly informative microsatellite marker sets for two marine serolid isopod species. For Serolis paradoxa (Fabricius, 1775), 13, and for Septemserolis septemcarinata (Miers, 1875), eight polymorphic microsatellite markers were isolated using the reporter genome enrichment protocol. The number of alleles per locus ( N A ) and the observed heterozygosity ( H O ) encompass a wide range of variation within S. paradoxa ( N A 3–31, H O 6–89%) and S. septemcarinata ( N A 2–18, H O 9–94%). The suitability of the newly isolated markers for population genetic studies is evaluated.