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Development of new microsatellite loci and multiplex reactions for muskellunge ( Esox masquinongy )
Author(s) -
SLOSS BRIAN L.,
FRANCKOWIAK RYAN P.,
MURPHY EDWARD L.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2007.02075.x
Subject(s) - esox , biology , pike , hatchery , microsatellite , multiplex polymerase chain reaction , population , range (aeronautics) , loss of heterozygosity , evolutionary biology , zoology , genetics , polymerase chain reaction , fishery , gene , demography , fish <actinopterygii> , allele , materials science , sociology , composite material
The muskellunge ( Esox masquinongy ) is a valued fisheries species throughout its native range. Numerous studies have documented performance and phenotypic differences among muskellunge populations, but genetic markers for assessment have been lacking. We characterized 14 microsatellite loci and developed five multiplex polymerase chain reactions. Successful amplification of northern pike ( Esox lucius ) was observed for seven loci. These microsatellites will be useful for analysing population structure, performance characteristics of propagated strains, and helping to develop and monitor hatchery management guidelines for muskellunge.