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A high‐throughput protocol for extracting high‐purity genomic DNA from plants and animals
Author(s) -
WHITLOCK R.,
HIPPERSON H.,
MANNARELLI M.,
BURKE T.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2007.02074.x
Subject(s) - biology , dna , genomic dna , dna extraction , chaotropic agent , extraction (chemistry) , computational biology , chromatography , genetics , biochemistry , polymerase chain reaction , gene , chemistry
DNA extraction techniques that employ the reversible binding of DNA to silica via chaotropic salts can deliver high‐quality genomic DNA from plant and animal tissues, while avoiding the use of toxic organic solvents. Existing techniques that use this method are either prohibitively expensive, or are applicable to only a restricted set of taxa. Here we describe a cost‐effective DNA extraction technique suitable for a wide range of plant and animal taxa that yields microgram quantities of high‐molecular‐weight genomic DNA at a throughput of 192 samples per day. Our technique is particularly robust for tissue samples that are insoluble or are rapidly discoloured or oxidized in standard DNA extraction buffers. We demonstrate the quality of DNA extracted using this method by applying the amplified fragment length polymorphism technique to plant species.