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Development of cytochrome b primers for mitotyping of barbels ( Barbus spp.)
Author(s) -
GANTE H. F.,
ALVES M. J.,
DOWLING T. E.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2007.02064.x
Subject(s) - barbel , biology , barbus , cytochrome b , phylogenetic tree , haplotype , population , genetics , zoology , evolutionary biology , gene , cyprinidae , genotype , fish <actinopterygii> , fishery , demography , sociology
Abstract Primers were developed that allow for rapid, reliable and inexpensive screening of cytochrome b by analysis of single‐stranded conformational polymorphisms. Twenty different haplotypes were identified from six species of Iberian Barbus . These primers proved useful for population and species level studies, and could also be valuable in population genetic and phylogenetic studies of other cyprinin fishes.