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Microsatellites from kousa dogwood ( Cornus kousa )
Author(s) -
WADL PHILLIP A.,
WANG XINWANG,
SCHEFFLER BRIAN E.,
RINEHART TIMOTHY A.,
TRIGIANO ROBERT N.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2007.02062.x
Subject(s) - biology , microsatellite , botany , genetics , gene , allele
Microsatellite loci were identified from Cornus kousa ‘National’. Primer pairs for 86 loci were developed and of these, eight were optimized and screened using genomic DNA from 22 kousa cultivars. All optimized loci were polymorphic and the number of alleles per locus ranged from three to 17. Observed heterozygosity ranged from 0 to 0.3 and expected heterozygosity ranged from 0.38 to 0.91. These microsatellites will be useful in population studies, and a breeding programme for cultivar development of Cornus species.