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Isolation and characterization of polymorphic microsatellite markers from Australia short‐finned eel ( Anguilla australis Richardson)
Author(s) -
GONG XIAOLING,
LI SIFA,
CAI WANQI,
WANG CHENGHUI
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2007.02060.x
Subject(s) - biology , loss of heterozygosity , microsatellite , locus (genetics) , linkage disequilibrium , polymerase chain reaction , genetics , population , allele , zoology , microbiology and biotechnology , gene , haplotype , demography , sociology
Seventeen microsatellite DNA loci from the Australian short‐finned eel ( Anguilla australis Richardson) were isolated and their amplification characteristics were described. The polymerase chain reaction primers were tested on 40 eel individuals. The primers amplified loci with relatively high numbers of alleles, ranging from five to 14 with an average of nine per locus. Mean observed heterozygosity ( H O ) and expected heterozygosity ( H E ) were 0.6779 and 0.7374, respectively, indicating that these markers would be useful for population studies. No loci deviated significantly from Hardy–Weinberg equilibrium ( P = 0.05) and no evidence was found for genotypic disequilibrium among loci at a 5% significance level.