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New polymorphic dinucleotide and trinucleotide microsatellite loci for hop Humulus lupulus L.
Author(s) -
JAKSE J.,
LUTHAR Z.,
JAVORNIK B.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2007.02053.x
Subject(s) - humulus lupulus , biology , microsatellite , loss of heterozygosity , genetics , locus (genetics) , hop (telecommunications) , allele , population , genotype , gene , horticulture , computer network , demography , pepper , sociology , computer science
One hundred and thirty‐five microsatellite markers were developed for hop Humulus lupulus L. from di‐ and trinucleotide‐enriched libraries. Seventy‐eight primers showed amplification in two tested genotypes. Twenty‐four loci were further characterized on a population of 34 hop samples and the number of alleles per locus, observed heterozygosity and expected heterozygosity ranged from two to 20 (9.7 on average), from 0.0294 to 0.9412 (0.6234 on average) and from 0.0294 to 0.9170 (0.6720 on average), respectively. These microsatellite markers will be further used for studying population structures and relationships and for identifying important qualitative and quantitative loci of hop.