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Cross‐species tests of 45 microsatellite loci isolated from different species of ungulates in the Iberian red deer ( Cervus elaphus hispanicus ) to generate a multiplex panel
Author(s) -
SANCHEZFERNANDEZ B.,
SORIGUER R.,
RICO C.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2007.02034.x
Subject(s) - biology , cervus elaphus , microsatellite , multiplex polymerase chain reaction , loss of heterozygosity , zoology , polymerase chain reaction , multiplex , cervus , genetics , allele , ecology , gene
The Iberian red deer ( Cervus elaphus hispanicus ) is an emblematic game species in Spain. To generate a battery of polymorphic markers for multiplex polymerase chain reactions for the Spanish red deer, 45 loci isolated in different species of ungulates were tested. Of the primers tested, 27 amplified but only 21 were polymorphic. Eleven of these markers were subsequently optimized for multiplex in four polymerase chain reactions. This allows analysing several molecular markers jointly to substantially reduce costs. Finally, we report descriptive summary statistics such as number of alleles for the former and also test of disequilibria and heterozygosity for the latter.