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Analysis of pigmented polymers in red wine by reverse phase HPLC
Author(s) -
PENG Z.,
ILAND P.G.,
OBERHOLSTER A.,
SEFTON M.A.,
WATERS E.J.
Publication year - 2002
Publication title -
australian journal of grape and wine research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.65
H-Index - 77
eISSN - 1755-0238
pISSN - 1322-7130
DOI - 10.1111/j.1755-0238.2002.tb00213.x
Subject(s) - wine , chromatography , polymer , chemistry , high performance liquid chromatography , pigment , ultrafiltration (renal) , wine color , organic chemistry , food science
A reverse phase HPLC method has been developed for the quantitative analysis of pigmented poly‐phenolic polymers in red wines. Pigmented polymers were well separated from anthocyanins and other simple wine pigments and eluted last as a single peak. The polymeric and polyphenolic nature of this peak was confirmed by its precipitation with gelatine and its behaviour during ultrafiltration and chromato‐graphy on Sephadex LH20. This method correlates well with spectrophotometric measures of total pigmented polymers and indices of wine age. The proportion of the pigmented material incorporated into polymers increased with increasing wine age.