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The immunoperoxidase method for the rapid diagnosis of intraoral herpes simplex virus infection in patients receiving bone marrow transplants
Author(s) -
FLAITZ CATHERINE M.,
HAMMOND HAROLD L.
Publication year - 1988
Publication title -
special care in dentistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.328
H-Index - 41
eISSN - 1754-4505
pISSN - 0275-1879
DOI - 10.1111/j.1754-4505.1988.tb00698.x
Subject(s) - immunoperoxidase , papanicolaou stain , medicine , herpes simplex virus , pathology , exfoliative cytology , herpes genitalis , hsl and hsv , bone marrow , viral culture , cytology , staining , multinucleate , virus , virology , immunology , antibody , cervical cancer , cancer , monoclonal antibody , genital herpes
Bone marrow transplant patients (N = 12) with oral lesions consistent with herpes simplex virus (HSV) infection were studied to compare the sensitivity of two rapid cytodiagnostic methods with viral cultures. Routine viral cultures and exfoliative cytology smears were prepared for microscopic evaluation using the Papanicolaou method and an immmunoperoxidase staining technique for detection of HSV type 1. Patients with a positive viral culture (N = 10) showed positive staining for HSV, using the immunoperoxidase technique. Multinucleated giant epithelial cells were observed in only seven of the ten positive specimens, using the Papanicolaou method. The results from this preliminary study suggest that the immunoperoxidase method may provide the dentist with a reliable and rapid approach for the diagnosis of intraoral HSV.