Open AccessEvolved polygenic herbicide resistance in L olium rigidum by low‐dose herbicide selection within standing genetic variation
Author(s) -
Busi Roberto,
Neve Paul,
Powles Stephen
Publication year - 2013
Publication title -
evolutionary applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.776
H-Index - 68
ISSN - 1752-4571
DOI - 10.1111/j.1752-4571.2012.00282.x
Subject(s) - lolium rigidum , biology , quantitative trait locus , polygene , genetics , population , genetic variation , cross resistance , mendelian inheritance , additive genetic effects , herbicide resistance , selection (genetic algorithm) , gene , dominance (genetics) , resistance (ecology) , heritability , agronomy , demography , artificial intelligence , sociology , computer science
The interaction between environment and genetic traits under selection is the basis of evolution. In this study, we have investigated the genetic basis of herbicide resistance in a highly characterized initially herbicide‐susceptible L olium rigidum population recurrently selected with low (below recommended label) doses of the herbicide diclofop‐methyl. We report the variability in herbicide resistance levels observed in F 1 families and the segregation of resistance observed in F 2 and back‐cross ( BC ) families. The selected herbicide resistance phenotypic trait(s) appear to be under complex polygenic control. The estimation of the effective minimum number of genes ( N E ), depending on the herbicide dose used, reveals at least three resistance genes had been enriched. A joint scaling test indicates that an additive‐dominance model best explains gene interactions in parental, F 1 , F 2 and BC families. The M endelian study of six F 2 and two BC segregating families confirmed involvement of more than one resistance gene. Cross‐pollinated L . rigidum under selection at low herbicide dose can rapidly evolve polygenic broad‐spectrum herbicide resistance by quantitative accumulation of additive genes of small effect. This can be minimized by using herbicides at the recommended dose which causes high mortality acting outside the normal range of phenotypic variation for herbicide susceptibility.