Open AccessIn situ TCE degradation mediated by complex dehalorespiring communities during biostimulation processes
Author(s) -
DugatBony Eric,
BiderrePetit Corinne,
Jaziri Faouzi,
David Maude M.,
Defoux Jérémie,
Lyon Delina Y.,
Richard JeanYves,
Curvers Cyrille,
Boucher Delphine,
Vogel Timothy M.,
Peyretaillade Eric,
Peyret Pierre
Publication year - 2012
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/j.1751-7915.2012.00339.x
Subject(s) - dehalococcoides , bioremediation , geobacter , biostimulation , reductive dechlorination , environmental chemistry , vinyl chloride , bioaugmentation , chemistry , contamination , biology , ecology , bacteria , genetics , organic chemistry , biofilm , copolymer , polymer
Summary The bioremediation of chloroethene contaminants in groundwater polluted systems is still a serious environmental challenge. Many previous studies have shown that cooperation of several dechlorinators is crucial for complete dechlorination of trichloroethene to ethene. In the present study, we used an explorative functional DNA microarray (DechloArray) to examine the composition of specific functional genes in groundwater samples in which chloroethene bioremediation was enhanced by delivery of hydrogen‐releasing compounds. Our results demonstrate for the first time that complete biodegradation occurs through spatial and temporal variations of a wide diversity of dehalorespiring populations involving both Sulfurospirillum, Dehalobacter , Desulfitobacterium , Geobacter and Dehalococcoides genera. Sulfurospirillum appears to be the most active in the highly contaminated source zone, while Geobacter was only detected in the slightly contaminated downstream zone. The concomitant detection of both bvcA and vcrA genes suggests that at least two different Dehalococcoides species are probably responsible for the dechlorination of dichloroethenes and vinyl chloride to ethene. These species were not detected on sites where cis ‐dichloroethene accumulation was observed. These results support the notion that monitoring dechlorinators by the presence of specific functional biomarkers using a powerful tool such as DechloArray will be useful for surveying the efficiency of bioremediation strategies.